Can the EF600 be used to thaw cells?
It has been shown (Morris et al., 2007) that the EF600 can be used for the controlled thawing of cells. Straws cooled to liquid nitrogen temperatures were placed on the sample plate of the EF600 maintained at -100°C and warmed at linear rates to +20°C. The linear rates of warming that are possible are relatively slow (<2°C min) and are mainly of interest for research applications. There are a few applications; for example the thawing of mammalian embryos which have been cooled slowly (0.3 °C/min) to -70°C and which then require slow thawing to allow recovery. However with the standard embryo protocols, as programmed into the EF600, rapid thawing is advisable.
The potential of using the EF600, operating isothermally at 37°C, to allow controlled thawing of cryopreserved samples in clean rooms in being examined.
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