EF600 FAQs

See the Asymptote guide to cryopreservation to learn about the physical processes underlying cryopreservation. Measured performance of the various EF600 devices are described elsewhere.  Specific questions raised about the EF600;

How does the Stirling engine work?

Why controlled rate freezing?

Is ice nucleation essential?

How is ice nucleation performed in clean rooms?

The minimum operating temperature of the EF600 is -100C is this a problem?

Must samples be cooled at a controlled rate to below -132°C - the "glass transition temperature of water"?

Is vitrification an alternative to standard cryopreservation (slow cooling)?

Why don't you use a probe directly in a reference sample to control the temperature during freezing in the EF600?

Is sticking of straws and cryovials to the sample plate a problem? 

Can 0.25 ml straws be used in the EF600-100?

What cryovials are compatible with the EF600-102?

Can the EF600 be used to thaw cells?

What are the noise levels for the EF600?

The EF600 comes with pre-set programmes – can these be edited?