Freeze fracture electron microscopy of an aqueous solution of glycerol (10%) frozen in a straw (0.25ml capacity). Ice was nucleated within the straw at -7°C. The sample was then cooled at 10°C per minute to -100°C and then transferred to liquid nitrogen. Longitudinal fracture of the straw following by deep etching reveals the structure of ice (etched areas) and of the freeze concentrated material (non-etched material). The structure of the ice along the straw shows evidence of the longitudinal growth of ice following nucleation. Low power. For higher magnifications of this sample see the next pages. Also, Compare with a cross fracture of a straw frozen under the same conditions.
